Burn Wound Healing Effect and Hair Growth Promoting Activity of Lawsonia inermis L. and Honey in Oryctolagus cuniculus Rabbits

Abstract

This study aimed to assess Lawsonia inermis L. and honey mixture effects on burn wounds and hair growth in rabbits. Nine male Oryctolagus cuniculus local rabbits were allocated randomly in 3 groups. After antisepsis and local anesthesia, four circular burns were created on the animal’s backs. Immediately after burning, the wounds were covered with honey in three rabbits (HON group), another group was treated first with honey and then L. inermis powder was added on honey (LI_HON group). Three untreated animals were used as control (CRL group). The treatment of rabbits was applied once daily (6/7 days) until 24th day post burns. The animals were observed for their general state, aspects of wounds and the healing times were also recorded. At 35th day post burns, the hair growth was investigated in the different groups. LI_HON group has recorded the best results compared to other groups; a short significant necrosis duration (LI_HON Vs CRL, p<0.001; LI_Hon Vs HON, p<0.05), precocious start of 1st crust detachment (LI_HON Vs CRL, p<0.05; LI_Hon Vs HON, p<0.001), a quick complete detachment of the first crust (LI_HON Vs CRL, p<0.05; LI_Hon Vs HON, p<0.05) and a significant reduction of healing time (LI_HON Vs CRL, p<0.05; LI_Hon Vs HON, p<0.01). LI-HON group has shown the best promoting activity of hair growth in term of recovered surface percentage (LI_HON Vs CRL, p = 9,13038E^-7; LI_Hon Vs HON, P = 1,70745E^-7) and hair length (LI_HON Vs CRL, p<0.01; LI_Hon Vs HON, p≤0.001). The study concludes that the topic use of L. inermis powder and honey simultaneously accelerates burn wound healing process in rabbit’s model. Henna has also shown a remarkable hair growth promoting activity in term of hair recovered area and hair length.