Research Article Open Access

Electrophoretic Identification of Casein in Various Types of Milk

Makhpal Yelubaevna Yelubaeva1, Shynar Yırmkızy Kenenbay2, Mirash Hudretovna Narmuratova3, Assiya Demeukhanovna Serikbayeva1 and Batyrkhan Azimkhanovich Buralkhiev1
  • 1 The Kazakh National Agrarian University, 8, Abay Avenue, Almaty, Kazakhstan, 050008, Kazakhstan
  • 2 The Almaty Technological University, 100, Tole bi, Almaty, Kazakhstan, 050012, Kazakhstan
  • 3 The Al-Farabi Kazakh National University, 71, al-Farabi Ave. Almaty, Kazakhstan, 050040, Kazakhstan

Abstract

Casein proteins of camel milk were obtained from skimmed milk by sedimentation (pH 4.6) at 24°C. To sediment proteins from mare milk, pH was increased to 4.6 and milk was centrifuged at 45,000 rpm for 30 minutes at 4°C. The supernatant obtained after centrifugation was heated up to 30°C for further fractionation of whey proteins. The sedimented casein from camel and mare milk was cleaned with chromatography. Casein fractions were analyzed with the use of electrophoresis based on SDS-Na polyacrylamide. This analysis showed the presence of α- , β- and κ-caseins, as well as fractions of whey proteins, such as α-lactalbumin and β-lactoglobulin in various types of milk and fermented products. Comparative study by the content of protein component of the casein complex in camel milk showed the following: αs-casein 31.5%, β-casein 64.5%, κ-casein 4%, in cow milk 40.12%, 24.28%, 27.93%, respectively and γ-casein amounts to 7.67%.

OnLine Journal of Biological Sciences
Volume 17 No. 4, 2017, 348-352

DOI: https://doi.org/10.3844/ojbsci.2017.348.352

Submitted On: 8 August 2017 Published On: 1 December 2017

How to Cite: Yelubaeva, M. Y., Kenenbay, S. Y., Narmuratova, M. H., Serikbayeva, A. D. & Buralkhiev, B. A. (2017). Electrophoretic Identification of Casein in Various Types of Milk. OnLine Journal of Biological Sciences, 17(4), 348-352. https://doi.org/10.3844/ojbsci.2017.348.352

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Keywords

  • Proteins
  • Casein
  • Whey Proteins
  • Camel Milk
  • Mare Milk