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ISSN: 1608-4217 (Print)
ISSN: 2410-8561 (Online)
Research Article Open Access

Flavonoids and Antimicrobial Properties of Begonia fischeri var. palustris in vitro Plantlets

Evgeniya A. Karpova1, Alexandra Yu. Nabieva1, Tatiana D. Fershalova1, Yuliya L. Yakimova2 and Nataliya V. Tsybulya1
  • 1 Central Siberian Botanical Garden of the Siberian Branch of Russian Academy of Sciences (CSBG RAS), Novosibirsk, Russia
  • 2 State Research Center of Virology and Biotechnology VECTOR, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia

Abstract

The objective of this study was to design a protocol of the successful establishment of plants of Begonia fischeri var. palustris obtained in the in vitro seeds culture, to evaluate flavonoid content and antimicrobial properties of in vitro plantlets. The significant increase of percentage of seed germination (92.5%) was recorded in the half strength MS medium and 1% agar medium (83.3%) in comparison with 50-60% in greenhouse conditions. Flavonoid composition of the leaves of in vitro plantlets and greenhouse stock plants had no substantial differences. Significant differences (p<0.05) were not found between flavonoid contents of the leaves (13.6 and 15.5 mg•g-1 of Dry Weight (DW), respectively). Aqueous ethanol extracts of plants showed antimicrobial effects against reference strains of Bacillus subtilis, Streptococcus pyogenes and Staphylococcus aureus. Concentration of flavonoids in acetone and ethanol extracts of exudative compounds of the leaves of in vitro plantlets was 0.02 and 2.0 mg•g-1 DW, respectively. Our results indicated that this protocol can be used for the production of a large number of plants of B. fischeri var. palustris with rich flavonoid composition and high quantities of flavonoids, including exudative compounds, with healing and air cleaning properties.

OnLine Journal of Biological Sciences
Volume 19 No. 1, 2019, 20-27

DOI: https://doi.org/10.3844/ojbsci.2019.20.27

Submitted On: 13 December 2018 Published On: 29 January 2019

How to Cite: Karpova, E. A., Nabieva, A. Y., Fershalova, T. D., Yakimova, Y. L. & Tsybulya, N. V. (2019). Flavonoids and Antimicrobial Properties of Begonia fischeri var. palustris in vitro Plantlets. OnLine Journal of Biological Sciences, 19(1), 20-27. https://doi.org/10.3844/ojbsci.2019.20.27

Open AccessCopyright: © 2019 Evgeniya A. Karpova, Alexandra Yu. Nabieva, Tatiana D. Fershalova, Yuliya L. Yakimova and Nataliya V. Tsybulya. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Keywords

  • Antimicrobial Properties
  • Begonia fischeri var. palustris
  • Flavonoids
  • Indoor Plant Design
  • in vitro Seed Germination
  • Metabolic Engineering