FSH Receptor and Ki-67 Protein Expressions of Endometrial Macaque in Mid-Luteal Phase after Exposure to Various Doses of Recombinant FSH and Fixed-Dose of GnRH Agonist

Abstract

There are many advances in Assisted Reproductive Technologies (ARTs). However, implantation rates are still low. The embryo implantation is a mutual interaction between the blastocyst and endometrium. This study aimed to investigate the endometrial cell proliferation stimulated by recombinant Follicle-Stimulating Hormone (rFSH) in various doses by looking at FSH-Receptor (FSHR) and Ki-67 protein expressions. The studied samples were Stored Biological Materials (SBMs) from the endometrial tissue of Macaca nemestrina. All sample was obtained from fifteen macaques, divided into four groups, which three groups were treated with rFSH and given fixed-dose (160 µg/day) of Gonadotropin-Releasing Hormone (GnRH) agonists and one control group as normal group. The three treated macaque groups were stimulated with 30, 50 and 70 IU doses of rFSH. There was no significant difference in various doses of rFSH towards FSHR and Ki-67 expressions in endometrial tissue of M. nemestrina. Therefore, this result did not significantly correlate FSHR and Ki-67 expressions in the endometrial tissue of M. nemestrina after rFSH administration. Moreover, the highest rFSH dose did not reduce the FSHR and Ki-67 expressions and there was no correlation between FSHR and Ki-67 expressions. It can be concluded that macaque endometrial cells were receptive to the given dosages of rFSH.