Analysis of the Protein Profile of Cassava Plantlets (Manihot esculenta Crantz.) Resistance to Fusarium Wilt Disease

Abstract

Fusarium wilt disease is still a production constraint in Cassava (Manihot esculenta Crantz.) cultivation. The disease is caused by the fungus Fusarium oxysporum (Fo), which to date has not been cured. It is expected that the use of varieties of cassava that are resistant to Fusarium wilt is an important alternative for disease control. Induced Resistance cassava research on Murashige and Skoog medium containing Fusaric Acid (FA) selective concentration has been done before and there were indications FA concentration tolerant to the selection of resistant plantlets in vitro. It is hoped that cassava plantlets that are resistant to FA will also be resistant to Fo. In previous studies, the inoculation of Fo fungal isolates on resistant cassava plantlets was carried out in vitro, followed by DNA pattern analysis compared to controls. The results of the DNA pattern analysis, in the form of a new (specific) DNA band that has a size of 550 bp (OPA_1) and 300 bp (OPA_10), are predicted to be candidates for RAPD markers for cassava resistance to Fo. Based on the results of these previous studies, it is necessary to study more deeply in this study to ascertain whether the new DNA strand is really a peroxidase protein that causes cassava plantlets to be resistant to Fo by protein profile analysis. The results of protein profile analysis showed that the appearance of new protein bands (around 98 kD) indicated the formation of PR-protein (peroxidase) in cassava plantlets that were resistant to Fo and missing protein bands (around 65 kD) in cassava plantlets.