Research Article Open Access

Antioxidant, Analgesic and Anti-inflammatory Effects of Ethanolic Extract of Moroccan Lavandula stoechas L.

Hind Ferehan1, Mehdi Ait Laaradia2, Sara Oufquir2, Rachida Aboufatima3, Soad Moubtakir4, Zahra Sokar2, Abderrahman Chait2 and Abdellah Bagri1
  • 1 Hassan First University of Settat, Faculty of Sciences and Techniques, Laboratory of Biochemistry and Neuroscience, Integrative and Computational Neuroscience Team, 26002, Settat, Morocco
  • 2 Cadi Ayad University, Faculty of Sciences Semlalia, Laboratory of Neurobiology, Pharmacology, Anthropology and Environment, 40000, Marrakech, Morocco
  • 3 Sultan Moulay Slimane University, Faculty of Sciences and Technology, Laboratory of Biological Engineering, 23000, Beni Mellal, Morocco
  • 4 Cadi Ayad UniversityCadi Ayad University, Faculty of Sciences Semlalia, Laboratory of Neurobiology, Pharmacology, Anthropology and Environment, 40000, Marrakech, Morocco

Abstract

Lavandula stoechas L. is a widely used medicinal plant. In Moroccan traditional medicine, it is employed for the treatment of different illness and as an anxiolytic, analgesic or anti-inflammatory remedy. The purpose of this investigation was to assess the antioxidant, antinociceptive and anti-inflammatory effects of the ethanolic extract of Moroccan Lavandula stoechas L. in animal models. Therefore, in vitro anti-oxidant activity was appraised via two techniques, the 2,2-Diphenyl-1-Picryl-Hydrazyl (DPPH) radical scavenging method and reducing power approach. For the in vivo trials, the ethanolic extract of the herb was orally administered (0.5, 0.6 and 1 g/kg, p.o.). Furthermore, acetic acid induced-writhing, formalin induced-paw-licking, thermal hyperalgesia and tail-flick experiments were applied to examine the antalgic activity. The xylene induced ear-edema trial was achieved to estimate the anti-inflammatory activity. The results revealed an important anti-oxidant activity of our extract. Moreover, in the writhing-test, we observed that the concentrate inhibited abdominal contractions engendered by the acetic-acid in mice. In the hot-plate and the tail-flick tests, the extract caused a notable central analgesic reaction, the latency to the thermal stimuli intensified in a dose dependent way. Additionally, considering the formalin test, the pre-treatment with the extract reduced the licking-time in the first and late phase of the response. Lastly, the xylene induced ear-edema test demonstrated that the plant’s extract remarkably restrained the edema formation occasioned by the xylene, which was confirmed by histological verification. This study allowed us to conclude that Lavandula stoechas is an ultimate medicinal plant that possesses antioxidant and anti-inflammatory activities along with the central and peripheral anti nociceptive properties.

OnLine Journal of Biological Sciences
Volume 21 No. 4, 2021, 346-358

DOI: https://doi.org/10.3844/ojbsci.2021.346.358

Submitted On: 6 August 2021 Published On: 15 February 2022

How to Cite: Ferehan, H., Laaradia, M. A., Oufquir, S., Aboufatima, R., Moubtakir, S., Sokar, Z., Chait, A. & Bagri, A. (2021). Antioxidant, Analgesic and Anti-inflammatory Effects of Ethanolic Extract of Moroccan Lavandula stoechas L.. OnLine Journal of Biological Sciences, 21(4), 346-358. https://doi.org/10.3844/ojbsci.2021.346.358

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Keywords

  • Lavandula stoechas L.
  • Antioxidant Activity
  • Anti-Nociceptive Activity
  • Anti-Inflammatory Effect