Molecular Characterization and Differentiation of Proteases Isolated from Different Aspergillus Fungal Species
- 1 Department of Nucleic Acid Research, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, New Borg El-Arab City 21934, Alexandria, Egypt
Abstract
A comparative molecular study of Aperigiullus protease using sequence of protease gene representative 25 different Aspergillus species (Aspergillus fumigatus, Aspergillus oryzae, Aspergillus flavus, Aspergillus novofumigatus, Aspergillus viridinutans, Aspergillus pseudotamarii, Aspergillus clavatus, Aspergillus sojae, Aspergillus fischeri, Aspergillus caelatus, Aspergillus novofumigatus, Aspergillus campestris, Aspergillus costaricaensis, Aspergillus candidus, Aspergillus neoniger, Aspergillus mulundensis, Aspergillus ibericus, Aspergillus niger, Aspergillus piperis, Aspergillus eucalypticola, Aspergillus vadensis, Aspergillus steynii, and Aspergillus lentulus) have been described for several properties. To summarise the Asperigillus protease correlation, alignment of multiple sequence and phylogenetic tree assembly were carried out. 59 Protease gene amino acid sequence used for the constructed phylogenetic tree that showed the specific protease clusters of several species of Aspergillus (Aspergillus oryzae, Aspergillus fumigatus, Aspergillus clavatus, Aspergillus niger, Aspergillus nidulans, Aspergillus viridinutans, Aspergillus flavus and Aspergillus fischeri). Protease gene amino acid sequences acquired from NCBI. The homology sequence level was done using amino acid sequence. Among these proteases, the distributions of the commonly observed motifs were analysed. Protease protein sequences indicate that the structure and enzymatic function are involved.
DOI: https://doi.org/10.3844/ojbsci.2021.69.119
Copyright: © 2021 Samia Abd Allah Abdal-Aziz and Safaa M. Ali. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Keywords
- Protease
- Aspergillus
- Sequence
- Amino Acid
- Phylogenetic