Research Article Open Access

Preparation, Identification and Antioxidant Evaluation of Citrullus Colocynthis Root and Fruit Extracts Against Doxorubicin in Male Rats

Sarah Samir Othman1, Gamal M. Hamad2, Sabria Abo Zaid Hassan2, Eman Fayad3 and Safaa M. Ali4
  • 1 Department of Pharmaceutical Bioproducts Research, Genetic Engineering, and Biotechnology Research Institute, City of Scientific Research and Technological Applications, Egypt
  • 2 Department of Food Technology, Arid Lands and Cultivation Research Institute, City of Scientific Research and Technological Application, Egypt
  • 3 Department of Biotechnology, Faculty of Sciences, Taif University, South Africa
  • 4 Department of Nucleic Acid Research, Genetic Engineering, and Biotechnology Research Institute, City of Scientific Research and Technological Applications, Egypt

Abstract

Millions of people are affected by diseases in developing or developed countries. Citrullus colocynthis is a high-antioxidant wildflower, it is healthy to eat and may be used as a source of energy or medication. The goal of this research was to conduct a phytochemical analysis of Citrullus colocynthis fruit extract, which is abundant in Egypt. Different extracts from that plant were used, including root and fruit extracts. Biochemical analysis and various hematological parameters were among the parameters estimated. These aqueous extracts were also evaluated for their protective action on various antioxidants, renal and histological parameters and molecular changes of both extracts. According to the present findings, this plant can be used as an effective natural source of antioxidants and it was used to lessen oxidative stress's consequences in male rats produced by doxorubicin, a powerful chemotherapeutic drug used to treat a variety of cancers and known to generate oxidative stress. The results reveal that by increasing total antioxidant capacity, decreasing TBARS, increasing catalase, GPx, GST and improving molecular and histopathological changes, these extracts were able to lower the oxidative damage caused by this chemotherapeutic agent on the majority of measured parameters. Fruit extract was more efficient than root extract, highlighting the benefits of using such a powerful antioxidants, source as a nutritional supplement in patients suffering from a variety of diseases caused by various sources of oxidative stress, including chemotherapeutic agents, to help them to overcome their complications.

OnLine Journal of Biological Sciences
Volume 22 No. 1, 2022, 75-86

DOI: https://doi.org/10.3844/ojbsci.2022.75.86

Submitted On: 22 September 2021 Published On: 8 February 2022

How to Cite: Othman, S. S., Hamad, G. M., Zaid Hassan, S. A., Fayad, E. & Ali, S. M. (2022). Preparation, Identification and Antioxidant Evaluation of Citrullus Colocynthis Root and Fruit Extracts Against Doxorubicin in Male Rats. OnLine Journal of Biological Sciences, 22(1), 75-86. https://doi.org/10.3844/ojbsci.2022.75.86

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Keywords

  • Citrullus Colocynthis
  • Doxorubicin
  • Antioxidants
  • Oxidative Stress
  • Histopathological
  • TNF