TY  - JOUR
AU  - Yang, Yueping 
AU  - Zhang, Yao 
AU  - Liu, Xinai 
AU  - Liu, Zhuo 
AU  - Sun, Yan 
AU  - Liu, Qing 
AU  - Song, Yuanda 
PY  - 2025
TI  - Expression and Induction Optimization of Mucor Circinelloides Lipase in Escherichia Coli
JF  - American Journal of Biochemistry and Biotechnology
VL  - 21
IS  - 2
DO  - 10.3844/ajbbsp.2025.149.156
UR  - https://thescipub.com/abstract/ajbbsp.2025.149.156
AB  - Microbial lipases serve as versatile biocatalysts for modifying lipid substrates, enabling diverse biotechnological applications spanning food technology, renewable energy production, and pharmaceutical sectors. Genomic analysis of Mucor circinelloides WJ11 revealed multiple lipase-encoding genes, with WJ_23 successfully cloned and heterologously expressed in Escherichia coli. Since the lipase expression was mainly concentrated in the cell pellets, appropriate induction strategies had been investigated to enhance the extracellular production of the lipase. The results showed that when 0.5 mmol/L Isopropyl-1-thio-β-D-Galactopyranoside (IPTG) and 0.8% Triton X-100 were simultaneously added in the later stage of logarithmic growth at 30°C, the peak extracellular lipase activity attained2580 U/L, demonstrating a 6.79-fold increase over the wild strain. This study provided a theoretical basis for further improving the extracellular production of other heterogonous enzymes in E. coli, and it also laid a foundation for the future application of lipase in M. circinelloides.